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Related Concept Videos

CRISPR01:59

CRISPR

50.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Related Experiment Video

Updated: Jun 17, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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Harnessing the evolving CRISPR/Cas9 for precision oncology.

Tianye Li1,2, Shuiquan Li3, Yue Kang4

  • 1Department of Gynecology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310009, People's Republic of China.

Journal of Translational Medicine
|August 8, 2024
PubMed
Summary
This summary is machine-generated.

The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 gene editing system offers precise DNA manipulation for understanding and treating diseases like cancer. Ongoing innovations aim to overcome challenges for its clinical application in precision oncology.

Keywords:
Base editorCRISPR/Cas9Chimeric antigen receptorPrecision oncologySpCas9dCas9

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Area of Science:

  • Genetics and Molecular Biology
  • Biotechnology
  • Oncology

Background:

  • The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 system, derived from microbial immunity, provides precise, versatile DNA editing capabilities.
  • Its application has expanded beyond basic research, leading to therapeutic advancements such as CASGEVY™ for sickle cell anemia.

Purpose of the Study:

  • To explore the multifaceted applications of CRISPR/Cas9 in understanding cancer biology, from gene function investigation to disease modeling.
  • To highlight CRISPR/Cas9's role in developing novel, personalized cancer therapies and enhancing immunotherapies like CAR-T.
  • To discuss the potential of CRISPR/Cas9 in pre-emptive gene screening for cancer intervention.

Main Methods:

  • Utilizing CRISPR/Cas9 for precise gene manipulation in mammalian systems.
  • Employing CRISPR/Cas9 to model various cancer types and study tumor biology, including resistance and immune evasion.
  • Leveraging CRISPR/Cas9 for screening cancer-susceptible genes and enhancing targeted cancer therapies.

Main Results:

  • CRISPR/Cas9 facilitates a deeper understanding of cancer genetics and biology.
  • The system enables the development of targeted cancer treatments and improved CAR-T therapies.
  • CRISPR/Cas9 aids in identifying genes for early cancer intervention strategies.

Conclusions:

  • CRISPR/Cas9 is a transformative tool revolutionizing cancer research and treatment paradigms.
  • Despite challenges in efficacy, efficiency, and safety, continuous technological innovation is crucial.
  • The integration of CRISPR/Cas9 into clinical practice heralds a new era of precision oncology with potential for curative therapies.