Three-dimensional structural and metric characterisation of cardioids
Stefan H Geyer1, Lavinia Ceci Ginistrelli2, Tobias Ilmer2
1Division of Anatomy, Center for Anatomy and Cell Biology, MIC, Medical University of Vienna, Vienna, Austria.
Frontiers in Cell and Developmental Biology
|August 9, 2024
Summary
Scanning High Resolution Episcopic Microscopy (S-HREM) provides precise 3D structural data for developing cardioids. This technique aids in optimizing organoid generation and understanding structural remodeling in cardiac models.
Area of Science:
- Biomedical Engineering
- Developmental Biology
- Microscopy
Background:
- Accurate 3D structural data is crucial for organoid development and research.
- Existing methods may lack the resolution or volumetric capabilities needed for complex organoid structures.
Purpose of the Study:
- To establish and validate a 3D imaging technique for detailed analysis of cardioids.
- To provide precise volumetric and structural information for native and experimentally challenged cardioids.
Main Methods:
- Utilized Scanning High Resolution Episcopic Microscopy (S-HREM), a variant of HREM.
- Captured high-resolution images of resin block surfaces to create digital volume data with sub-micrometer voxel sizes.
- Analyzed native and cryo-injured cardioids at different developmental stages (day 10 and day 14).
Main Results:
- Generated precise volumetric data and detailed micro- and macroanatomic structural comparisons.
- Described the 3D arrangement and changes in cavity lining between developmental stages.
- Mapped cell types to their specific spatial and structural environments.
- Quantified injured areas in cryo-injured cardioids.
Conclusions:
- S-HREM produces high-quality digital volume data with significant potential for biological research.
- The technique provides essential metric and structural insights for cardioid generation and remodeling studies.
- This imaging approach enhances the interpretation of structural changes in cardiac organoid models.
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