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Updated: Jun 17, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

FAST-EM array tomography: a workflow for multibeam volume electron microscopy.

Arent J Kievits¹, B H Peter Duinkerken², Ryan Lane¹

¹Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands.

Methods in Microscopy

|August 9, 2024

Summary

This summary is machine-generated.

FAST-EM, a novel multibeam scanning transmission electron microscope, accelerates 3D nanoscale imaging of biological samples. This new workflow enables high-resolution, large-volume ultrastructural data acquisition within practical timeframes.

Keywords:

FAST-EM array tomography automatic segmentation image processing volume electron microscopy

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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Area of Science:

Cell Biology
Microscopy
Biophysics

Background:

Understanding complex biological processes requires high-resolution 3D nanoscale imaging of cells and tissues.
Traditional electron microscopy (EM) provides necessary resolution but suffers from low throughput, limiting large-volume imaging.
Overcoming throughput limitations is crucial for advancing biological research using EM.

Purpose of the Study:

To introduce a novel workflow for high-throughput volume electron microscopy (EM).
To present FAST-EM, a multibeam scanning transmission electron microscope designed for parallel data acquisition.
To demonstrate the capability of FAST-EM for reconstructing large, high-resolution 3D ultrastructural datasets.

Main Methods:

Development and implementation of FAST-EM, a 64-beam scanning transmission electron microscope.
Utilizing optical detection to separate signals from parallel electron beams.
Establishing a workflow for parallel acquisition and 3D reconstruction of ultrastructural data.

Main Results:

Demonstrated parallel acquisition of ultrastructural data using 64 electron beams.
Successfully performed 3D reconstruction of multiple biological samples.
Achieved large reconstructed volumes with high resolution and contrast.

Conclusions:

The FAST-EM workflow significantly enhances throughput for volume EM.
Enables high-resolution, large-scale 3D imaging of biological ultrastructure within feasible timeframes.
Addresses key limitations in current EM techniques for biological research.