In vitro lymphokine release by lymphocytes from mice infected with Salmonella

Lymphocytes generated in mice by infection with Salmonella enteritidis 11RX release interleukin 2 and macrophage activation factor upon subsequent in vitro culture with bacterial antigens. Lymphokine release requires the co-culture of non-adherent sensitized Lyt 1+2- T cells and adherent metabolically active accessory cells; the interaction between these two populations is restricted by the H-2 I-A locus. Following systemic immunization with the 11RX strain, the two lymphokines are produced in parallel by peritoneal cells, whereas spleen cells primarily release macrophage activation factor.