Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Generation of Action Potential in Skeletal Muscles

Generation of Action Potential in Skeletal Muscles

Every cell in the body maintains a membrane potential due to an uneven distribution of positive and negative charges across its plasma membrane. The membrane potential is measured in millivolts and quantifies the difference in charge across the membrane.
Like neurons, muscle cells are also regarded as excitable due to their capacity to change in response to stimuli, primarily due to voltage-gated ion channels embedded in their plasma membranes, which get activated by alterations in the...

Cross-bridge Cycle

Cross-bridge Cycle

As muscle contracts, the overlap between the thin and thick filaments increases, decreasing the length of the sarcomere—the contractile unit of the muscle—using energy in the form of ATP. At the molecular level, this is a cyclic, multistep process that involves binding and hydrolysis of ATP, and movement of actin by myosin.

Satellite Stem Cells and Muscular Dystrophy

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Acute kidney injury disrupts cardiac remodeling via SerpinA3N.

bioRxiv : the preprint server for biology·2025

Same author

Phenotypic screens reveal Plasmodium falciparum genetic factors associated with infection of sickle-trait cells.

Blood cells, molecules & diseases·2025

Same author

Supersaturation mutagenesis reveals adaptive rewiring of essential genes among malaria parasites.

Science (New York, N.Y.)·2025

Same author

Genetic factors regulating Plasmodium falciparum gametocytogenesis identified by phenotypic screens.

Scientific reports·2024

Same author

Elritercept, a modified activin receptor IIA ligand trap, increased erythropoiesis and thrombopoiesis in a phase 1 trial.

Blood advances·2024

Same author

IL-12 and PD-1 peptide combination gene therapy for the treatment of melanoma.

Molecular therapy. Nucleic acids·2024

Search research articles

Related Experiment Video

Updated: Jun 17, 2025

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

Published on: April 6, 2022

DNA Electrotransfer Regulates Molecular Functions in Skeletal Muscle.

Amanda Sales Conniff¹, Jared Tur¹, Kristopher Kohena¹

¹Department of Medical Engineering, University of South Florida, Tampa, Florida, USA.

|August 9, 2024

Summary

This summary is machine-generated.

Electric pulses enhance plasmid DNA delivery to muscle tissue, causing specific molecular changes related to stress and inflammation. Understanding these effects is key for developing effective gene therapies.

Keywords:

RNA sequencing electroporation electrotransfer molecular functions plasmid skeletal muscle

More Related Videos

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Published on: October 19, 2009

DNA Electroporation, Isolation and Imaging of Myofibers

DNA Electroporation, Isolation and Imaging of Myofibers

Published on: December 23, 2015

Related Experiment Videos

Last Updated: Jun 17, 2025

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

Published on: April 6, 2022

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Published on: October 19, 2009

DNA Electroporation, Isolation and Imaging of Myofibers

DNA Electroporation, Isolation and Imaging of Myofibers

Published on: December 23, 2015

Area of Science:

Molecular biology
Genetics
Biotechnology

Background:

Skeletal muscle is a target for plasmid DNA (pDNA) delivery for vaccines and therapeutics.
Electroporation (electric pulses) enhances cell permeability for improved pDNA delivery and expression.
Molecular effects of DNA electrotransfer on muscle tissue are not well understood.

Purpose of the Study:

To characterize the molecular changes in muscle tissue following intramuscular DNA electrotransfer.
To differentiate the effects of electric pulses versus pDNA injection on gene expression.
To identify unique molecular responses to guide gene therapy development.

Main Methods:

Intramuscular pDNA electrotransfer in muscle tissue.
RNA sequencing to evaluate gene expression changes four hours post-treatment.
Gene Ontology (GO) pathway enrichment analysis of differentially expressed genes.

Main Results:

GO analysis revealed enriched functions related to muscle stress, cytoskeleton, and inflammation from pulse application.
pDNA injection regulated terms associated with DNA-directed responses and control.
pDNA electrotransfer affected pathways similar to pulse application, but also included pDNA entry and trafficking.

Conclusions:

Muscle stimulus via DNA electrotransfer induces specific molecular functions.
Identifying these intrinsic molecular changes is crucial for designing effective gene therapies.