Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Immunocompetence01:22

Development of Immunocompetence

296
The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
296
Transcytosis of IgG01:15

Transcytosis of IgG

2.7K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
2.7K
Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

911
Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
911
Introduction to Innate and Adaptive Immunity01:21

Introduction to Innate and Adaptive Immunity

3.8K
The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...
3.8K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

67.9K
Overview
67.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hemoglobin alpha-like subunit mu is expressed during ontogeny and is elevated in alpha thalassemia.

Blood. Red cells & iron·2026
Same author

CSF1R Inhibition with Chemotherapy Relieves Systemic Immune Suppression in Patients with Metastatic Triple-Negative Breast Cancer and Boosts anti-PD-1 Efficacy in Transgenic Mammary Tumors.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Placental ferroptosis and impaired fetal growth in symptomatic and asymptomatic SARS-CoV-2 infections.

Clinical science (London, England : 1979)·2026
Same author

Imaging Mass Cytometry Immune Profiling of Hunner Lesions in a Convenience Sample of Patients With Interstitial Cystitis/Bladder Pain Syndrome.

The Journal of urology·2025
Same author

Adverse neonatal outcomes are associated with a sex-specific placental inflammatory profile†.

Biology of reproduction·2025
Same author

Perinatal Immune Changes to Identify Patients at Risk of Postpartum Preeclampsia.

American journal of hypertension·2025

Related Experiment Video

Updated: Jun 17, 2025

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
07:51

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Published on: May 21, 2015

17.2K

Maternal Innate Immune Reprogramming After Complicated Pregnancy.

Nakeisha A Lodge-Tulloch1, Jean-François Paré1, Camille Couture2,3,4

  • 1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.

American Journal of Reproductive Immunology (New York, N.Y. : 1989)
|August 9, 2024
PubMed
Summary

Women with preeclampsia (PE) and fetal growth restriction (FGR) show immune tolerance in monocytes before labor. This immune reprogramming may link pregnancy complications to later cardiovascular and metabolic disease risks.

Keywords:
epigenomicsfetal growth restrictionmonocytepreeclampsiasingle‐cell RNA sequence analysistolerancetrained immunity

More Related Videos

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
09:02

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Published on: October 14, 2021

4.7K
Isolation of Leukocytes from the Human Maternal-fetal Interface
08:19

Isolation of Leukocytes from the Human Maternal-fetal Interface

Published on: May 21, 2015

16.0K

Related Experiment Videos

Last Updated: Jun 17, 2025

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
07:51

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Published on: May 21, 2015

17.2K
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
09:02

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Published on: October 14, 2021

4.7K
Isolation of Leukocytes from the Human Maternal-fetal Interface
08:19

Isolation of Leukocytes from the Human Maternal-fetal Interface

Published on: May 21, 2015

16.0K

Area of Science:

  • Immunology
  • Reproductive Medicine
  • Epigenetics

Background:

  • Preeclampsia (PE) and fetal growth restriction (FGR) are linked to maternal inflammation and increased future cardiovascular/metabolic disease risk.
  • Epigenetic reprogramming of innate immune cells may underlie this increased disease susceptibility.

Purpose of the Study:

  • To investigate the immune phenotype of circulating monocytes in women with PE and FGR before labor.
  • To explore potential epigenetic modifications in monocytes associated with these pregnancy complications.

Main Methods:

  • Monocytes were isolated from women with PE, FGR, and uncomplicated pregnancies before labor.
  • Cytokine release (IL-10, IL-1β, GM-CSF, TNFα) after lipopolysaccharide (LPS) stimulation was measured.
  • Histone modification (H3K4me3) in TNF promoter regions and single-cell transcriptomic profiles were analyzed.

Main Results:

  • Monocytes from women with PE/FGR displayed altered cytokine secretion (increased IL-10, decreased IL-1β/GM-CSF) post-LPS.
  • Decreased H3K4me3 levels were observed in TNF promoter regions of monocytes from complicated pregnancies.
  • Transcriptomic analysis revealed a higher proportion of anti-inflammatory myeloid cells and fewer inflammatory non-classical monocytes in PE.

Conclusions:

  • Monocytes from women with PE and FGR exhibit an immune tolerance phenotype prior to labor.
  • Further research is needed to determine if this phenotype persists postpartum and contributes to long-term disease risk.