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Comparative global profiling of Perilla leaf and stem via transcriptomics and metabolomics
Fangyu Xing1, Qianlin Xiao1, Hameed Gul1
1College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, PR China.
Gene
|August 9, 2024
Summary
This study reveals distinct gene and metabolite profiles in Perilla stems and leaves, identifying key pathways like flavonoid biosynthesis in leaves for medicinal compound enhancement.
Area of Science:
- Plant Science
- Genomics
- Metabolomics
Background:
- Perilla (Perilla frutescens L.) is a globally utilized medicinal and culinary herb.
- Understanding tissue-specific compound production is crucial for optimizing yields in medicinal plants.
Purpose of the Study:
- To compare gene expression and metabolite profiles in Perilla stem (PfST) and leaf (PfLE) tissues.
- To identify tissue-specific molecular pathways and compounds for potential medicinal applications.
Main Methods:
- Genome-wide RNA-sequencing (RNA-seq) for transcriptomics.
- Global metabolite profiling for metabolomics.
- Comparative analysis of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) between PfST and PfLE.
Main Results:
- Identified 18,490 DEGs and 144 DAMs with tissue-specific expression and accumulation.
- Discovered 14 significantly enriched metabolic pathways common to both transcriptomics and metabolomics data.
- Observed significantly higher expression of flavonoid biosynthesis genes in PfLE, suggesting greater flavonoid accumulation in leaves.
Conclusions:
- Perilla leaves exhibit distinct molecular characteristics, particularly in flavonoid metabolism, compared to stems.
- This research provides a foundational understanding of organ-specific molecular mechanisms in Perilla.
- Findings can guide strategies for enhancing desirable medicinal compound production in Perilla and related species.
