Related Experiment Video
Updated: Jun 17, 2025

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
Published on: May 13, 2020
Star-Like Polypeptides as Simplified Analogues of Horseradish Peroxidase (HRP) Metalloenzymes
Antoine Tronnet1,2, Pedro Salas-Ambrosio1, Rosa Roman3
1CNRS, LCPO (Laboratoire de Chimie des Polymères Organiques (UMR5629)), University of Bordeaux, Bordeaux INP, 16 avenue Pey Berland, Pessac, F-33600, France.
Abstract:
Peroxidases, like horseradish peroxidase (HRP), are heme metalloenzymes that are powerful biocatalysts for various oxidation reactions. By using simple grafting-from approach, ring-opening polymerization (ROP), and manganese porphyrins, star-shaped polypeptides analogues of HRP capable of catalyzing oxidation reactions with H2O2 is successfully prepared. Like their protein model, these simplified analogues show interesting Michaelis-Menten constant (KM) in the mM range for the oxidant. Interestingly, the polymer structures are more resistant to denaturation (heat, proteolysis and oxidant concentration) than HRP, opening up interesting prospects for their use in catalysis or in biosensing devices.
Related Concept Videos
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
Radical Reactivity: Steric Effects
Along with electronic...
Ribozymes
Ribozymes can...
Catalytically Perfect Enzymes
Most enzymes...
Ligand Binding and Linkage
Structure of Porins

