Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

20.9K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
20.9K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

15.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.2K
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Guardians or Gateways? The Intricate Relationship Between Plant Cell Walls and Pathogenic Xanthomonas.

Molecular plant pathology·2026
Same author

<i>Clavibacter vidaverae</i> sp. nov., <i>Clavibacter rahimiani</i> sp. nov. and <i>Clavibacter davisi</i> sp. nov.: corynebacterial plant pathogens isolated from small-grain cereals.

International journal of systematic and evolutionary microbiology·2026
Same author

Lactuchelins represent lipopeptide siderophores produced by Pseudomonas lactucae that inhibit Xanthomonas campestris.

The ISME journal·2026
Same author

<i>Xanthomonas</i> coordinates type III-type II effector synergy by activating fruit-ripening pathway.

Science (New York, N.Y.)·2025
Same author

Dormancy and reactivation of the seed and its microbiome: a holobiont perspective.

mSystems·2025
Same author

The center of wheat domestication drives diversity of <i>Clavibacter</i> pathogens.

Applied and environmental microbiology·2025
Same journal

Antimicrobial resistance and genomic characteristics of bovine Escherichia coli from five northern provinces of China.

BMC genomics·2026
Same journal

Reference-guided transcriptomics resolves CoHMGR family composition and expression diversification during postharvest warm conditioning of Camellia oleifera seed kernels.

BMC genomics·2026
Same journal

Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.

BMC genomics·2026
Same journal

Comparative and population genomics analyses of eared pheasants inhabiting highly varying altitudes.

BMC genomics·2026
Same journal

Identification of differentially expressed lncRNAs in different daily weight gains of Jiangquan black pigs and functional analysis of LOC100518120.

BMC genomics·2026
Same journal

A self-attention-based deep learning model for identifying key genes in insect pupal metamorphosis.

BMC genomics·2026
See all related articles

Related Experiment Video

Updated: Jun 17, 2025

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
11:16

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Published on: July 22, 2017

14.0K

Comparative transcriptomics reveals a highly polymorphic Xanthomonas HrpG virulence regulon.

Thomas Quiroz Monnens1, Brice Roux1, Sébastien Cunnac2

  • 1LIPME, INRAE/CNRS UMR 0441/2594, Université de Toulouse, Université Paul Sabatier Toulouse 3, UMR, Castanet-Tolosan, 31320, France.

BMC Genomics
|August 9, 2024
PubMed
Summary
This summary is machine-generated.

HrpG regulates bacterial virulence in Xanthomonas crops. This study reveals diverse HrpG regulons across strains, showing varied strategies for virulence and adaptation beyond type III secretion systems.

Keywords:
XanthomonasHrpGRegulon diversityTranscriptome sequencingType III secretion system

More Related Videos

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
09:23

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

1.8K
A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
10:18

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Published on: April 25, 2015

10.4K

Related Experiment Videos

Last Updated: Jun 17, 2025

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
11:16

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Published on: July 22, 2017

14.0K
Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
09:23

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

1.8K
A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
10:18

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Published on: April 25, 2015

10.4K

Area of Science:

  • Microbiology
  • Plant Pathology
  • Bacterial Genetics

Background:

  • Xanthomonas bacteria cause significant crop diseases.
  • Virulence depends on type III effectors (T3Es) delivered by the type III secretion system (T3SS).
  • HrpG is a master regulator of T3SS, but its broader regulon is understudied.

Purpose of the Study:

  • To investigate the HrpG regulon across diverse Xanthomonas species.
  • To understand HrpG's role beyond T3SS regulation.

Main Methods:

  • Transcriptome sequencing of 17 Xanthomonas strains.
  • Constitutive expression of an active HrpG form (HrpG*) to induce regulon.

Main Results:

  • Significant diversity in HrpG* regulons was observed between and within Xanthomonas species.
  • The core HrpG* regulon includes T3SS genes, HrpX, T3Es XopR and XopL, an MFS transporter, and PhoC.
  • Variable regulation of chemotaxis and carbohydrate/proteolytic enzyme genes by HrpG*.

Conclusions:

  • HrpG-dependent virulence employs diverse, strain-specific strategies.
  • HrpG regulates virulence and adaptation pathways beyond T3Es and T3SS.