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Related Concept Videos

Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
Tuberculosis01:23

Tuberculosis

Tuberculosis (TB) remains a significant global health concern, primarily targeting the lungs and spreading through airborne transmission. Infection begins when aerosolized droplet nuclei, expelled by an individual with active TB, are inhaled by another person. These microscopic particles carry Mycobacterium tuberculosis, the causative agent of TB. Upon reaching the alveoli, the bacilli are engulfed by alveolar macrophages. However, due to their specialized lipid-rich cell wall, these pathogens...

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Related Experiment Video

Updated: Jul 7, 2026

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
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Single-cell transcriptomic analysis reveals a decrease in the frequency of macrophage-RGS1high subsets in patients

Ying Jiang1, Xinqiang Zhang2, Bo Wang1

  • 1The Eighth Medical Center of Chinese PLA General Hospital, Beijing, 100091, People's Republic of China.

Molecular Medicine (Cambridge, Mass.)
|August 9, 2024
PubMed
Summary

A specific macrophage subset (macrophage-RGS1high) is decreased in osteoarticular tuberculosis (OTB). This subset may help diagnose OTB and monitor treatment effectiveness.

Keywords:
BiomarkerFerroptosisMacrophagesOsteoarticular tuberculosisscRNA

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Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
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Area of Science:

  • Immunology
  • Infectious Diseases
  • Genomics

Background:

  • Immune cell subsets play a role in Mycobacterium tuberculosis (MTB) infection.
  • The specific immune cell subsets involved in osteoarticular tuberculosis (OTB) are not well understood.
  • Single-cell RNA sequencing (scRNA-Seq) can elucidate immune cell phenotypes and functions.

Purpose of the Study:

  • To investigate the phenotypes and functions of immune cell subsets in patients with OTB using scRNA-Seq.
  • To identify potential biomarkers for OTB diagnosis and treatment monitoring.

Main Methods:

  • scRNA-Seq was performed on pathological and healthy adjacent tissues from OTB patients.
  • Unsupervised clustering and uniform manifold approximation and projection (UMAP) were used for cell subset identification and visualization.
  • Immunohistochemistry and flow cytometry were employed to validate findings in OTB, osteoarticular bacterial infection (OBI), and healthy control (HC) samples.

Main Results:

  • Thirteen distinct cell subsets were identified in OTB tissues.
  • The frequency of the macrophage-RGS1high subset was significantly decreased in OTB tissues, correlating with increased MTB susceptibility via ferroptosis.
  • Decreased macrophage-RGS1high subset frequency in OTB tissues and blood samples differentiated OTB patients from HCs and OBI patients.

Conclusions:

  • The macrophage-RGS1high subset is reduced in OTB patients and may increase with effective treatment.
  • The macrophage-RGS1high subset shows potential as a diagnostic biomarker for OTB.
  • This subset could also serve as a biomarker for monitoring the efficacy of OTB treatment.