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Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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Key Elements for Plant Nutrition

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.

Overview of Nitrogen Metabolism

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Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
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Related Experiment Video

Updated: Jun 17, 2025

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Probing Biological Nitrogen Fixation in Legumes Using Raman Spectroscopy.

Abdolabbas Jafari¹, Kritarth Seth², Armin Werner¹

¹Lincoln Agritech, Lincoln University, Lincoln 7647, New Zealand.

Sensors (Basel, Switzerland)

|August 10, 2024

Summary

This summary is machine-generated.

This study shows Raman spectroscopy can rapidly assess biological nitrogen fixation (BNF) in soybeans. This non-invasive method offers a practical alternative to expensive traditional techniques for sustainable agriculture.

Keywords:

biosystems chemometrics non-destructive pattern recognition precision agriculture rhizobia symbiosis

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MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

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Published on: November 1, 2010

Area of Science:

Agricultural Science
Biochemistry
Spectroscopy

Background:

Biological nitrogen fixation (BNF) is crucial for sustainable agriculture.
Current BNF quantification methods are costly and impractical.
A rapid, non-invasive assessment technique is needed.

Purpose of the Study:

To explore Raman spectroscopy for assessing soybean BNF activity.
To identify spectral signatures linked to BNF.
To develop a spectroscopic model for BNF quantification.

Main Methods:

Soybean plants were analyzed using Raman spectroscopy.
δ15N isotope ratio mass spectrometry (IRMS) provided reference BNF percentages.
Partial least squares regression (PLSR) and elastic net (Enet) were used for modeling.

Main Results:

Raman spectra showed distinct signatures associated with BNF.
A PLSR model explained 80% of the variation in BNF activity.
Enet regularization improved model specificity and identified key wavenumbers.

Conclusions:

Raman spectroscopy is a promising non-invasive tool for rapid BNF assessment in soybeans.
This technique can support sustainable agriculture by providing practical BNF monitoring.
Further research can refine spectroscopic methods for nitrogen management.