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A Low-Cost Handheld Centrifugal Microfluidic System for Multiplexed Visual Detection Based on Isothermal
Nan Wang1, Xiaobin Dong1, Yijie Zhou1
1Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Sensors (Basel, Switzerland)
|August 10, 2024
Summary
A new low-cost, handheld device enables multiplexed visual detection using recombinase polymerase amplification (RPA). This system offers rapid, on-site testing for multiple targets, including African swine fever virus (ASFV).
Area of Science:
- Biotechnology
- Microfluidics
- Molecular Diagnostics
Background:
- Point-of-care diagnostics require portable and cost-effective solutions.
- Multiplexed detection enhances diagnostic efficiency by analyzing multiple targets simultaneously.
- Recombinase polymerase amplification (RPA) offers rapid, isothermal nucleic acid amplification suitable for field applications.
Purpose of the Study:
- To develop a low-cost, handheld centrifugal microfluidic system for multiplexed visual detection.
- To integrate RPA amplification with visual detection in a portable device.
- To demonstrate the system's capability for detecting multiple targets, such as African swine fever virus (ASFV) genes.
Main Methods:
- Development of a compact centrifugal microfluidic chip with four parallel reaction units.
- Integration of heating, optical, rotation, and sensing modules into a handheld companion device.
- Utilized non-contact heating, magnetic sensing-controlled motor for mixing and centrifugation, and optimized visual fluorescence detection.
Main Results:
- The system successfully performed multiplexed RPA amplification and visual detection.
- Achieved a limit of detection (LOD) of 75 copies/test for ASFV genes, comparable to tube-based RPA.
- Demonstrated direct visual observation of results using eyes or a smartphone.
Conclusions:
- The developed handheld system provides a cost-effective and portable solution for multiplexed visual detection.
- This technology has potential for rapid, on-site diagnostics in resource-limited settings.
- The system enables efficient detection of multiple nucleic acid targets, including specific viral genes.
