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Related Experiment Videos

Serotype c Streptococcus mutans mutatable to lactate dehydrogenase deficiency.

S Abhyankar, H J Sandham, K H Chan

    Journal of Dental Research
    |November 1, 1985
    PubMed
    Summary

    Researchers created lactate-dehydrogenase-deficient mutants of *S. mutans* serotype c. These mutants maintained high cell yields, similar to parent strains, despite lacking LDH activity.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Streptococcus mutans (S. mutans) is a key cariogenic bacterium.
    • Lactate dehydrogenase (LDH) plays a crucial role in S. mutans' carbohydrate metabolism.
    • Previous studies focused on LDH-deficient mutants in serotype g, with limited data on serotype c.

    Purpose of the Study:

    • To generate and characterize lactate-dehydrogenase-deficient mutants of serotype c S. mutans.
    • To investigate the metabolic and growth characteristics of these novel mutants.
    • To compare the properties of serotype c LDH-deficient mutants with those of serotype g.

    Main Methods:

    • Utilized N-methyl-N'-nitro-N-nitrosoguanidine for mutagenesis of serotype c S. mutans strains.
    • Selected parent strains that produced high levels of ethanol, acetic acid, and acetoin, with low lactic acid production.
    • Assessed LDH-specific activity in cell-free extracts and evaluated molar growth yields and cell crop production.

    Main Results:

    • Successfully generated stable lactate-dehydrogenase-deficient mutants of serotype c S. mutans.
    • Mutants exhibited less than 1% of the parent strain's LDH-specific activity.
    • Serotype c mutants demonstrated molar growth yields comparable to parent strains.
    • Unlike serotype g mutants, serotype c mutants achieved similar cell crop yields as their parent strains.

    Conclusions:

    • Lactate dehydrogenase deficiency in serotype c S. mutans does not impair molar growth yield or cell crop production.
    • These findings highlight differences in metabolic regulation between serotype c and serotype g S. mutans.
    • LDH-deficient serotype c mutants are viable and retain significant growth potential, offering avenues for further metabolic studies.

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