Insulin-Activated Signaling Pathway and GLUT4 Membrane Translocation in hiPSC-Derived Cardiomyocytes.
Giulia Querio1, Susanna Antoniotti2, Renzo Levi2
1Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043 Orbassano, Italy.
International Journal of Molecular Sciences
|August 10, 2024
Summary
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) show an insulin response similar to adult cells. Analyzing membrane fractions is key to observing glucose transporter GLUT4 dynamics in these hiPSC-CM models for cardio-metabolic research.
Area of Science:
- Cardiology
- Stem Cell Biology
- Metabolic Research
Background:
- Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are valuable for studying cardiac pathophysiology.
- Understanding cardio-metabolic disorders requires defining postnatal metabolic phenotypes in hiPSC-CMs.
Purpose of the Study:
- To monitor the insulin response in hiPSC-CMs.
- To investigate glucose transporter GLUT4 dynamics in response to insulin.
- To validate hiPSC-CMs as a model for cardio-metabolic research.
Main Methods:
- Western blot analysis of total cell lysates and membrane fractions from hiPSC-CMs.
- Insulin stimulation and assessment of AKT, AS160 phosphorylation, and GLUT4 expression/translocation.
Main Results:
- Insulin treatment increased AKT and AS160 phosphorylation in hiPSC-CMs.
- Western blot of total lysates did not show changes in GLUT4 expression.
- Western blot of membrane fractions revealed insulin-induced GLUT4 translocation to the plasma membrane.
Conclusions:
- hiPSC-CMs exhibit an insulin response comparable to adult cardiomyocytes, including intracellular signaling and GLUT4 translocation.
- Analyzing membrane fractions is crucial for monitoring GLUT4 dynamics in hiPSC-CMs.
- hiPSC-CMs are a suitable cellular model for cardio-metabolic research.
Keywords:
GLUT4cardiac maturationcardiomyocytehiPSC-CMinsulinlactate mediummetabolismplasma membrane translocationpostnatal cardiac cellMore Related Videos
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