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Follicular Fluid Proteomic Analysis to Identify Predictive Markers of Normal Embryonic Development.

Janusz Przewocki1,2, Dominik Kossiński2, Adam Łukaszuk3

  • 1Institute of Mathematics, University of Gdansk, 80-308 Gdańsk, Poland.

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|August 10, 2024
PubMed
Summary

Analyzing human follicular fluid (hFFs) can predict oocyte quality. Specific proteins like IgA1hc and dickkopf-related protein 3 in hFFs may serve as biomarkers for pre-fertilization screening in infertility treatments.

Keywords:
dickkopf-related protein 3embryo qualityfollicular fluidheat shock cognate 71 kDa proteinimmunoglobulin heavy constant alpha 1keratin type II cytoskeletal 1 proteinmass spectrometryoocyte qualityproteomics

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Area of Science:

  • Reproductive biology and assisted reproductive technologies.

Background:

  • Societal trends like aging populations and delayed childbearing increase the importance of improving infertility treatments.
  • Human follicular fluid (hFF) contains biomarkers that may reflect oocyte developmental quality.
  • Assessing oocyte quality pre-fertilization is crucial for personalized in vitro fertilization (IVF) and embryo culture.

Purpose of the Study:

  • To investigate the proteomic profile of human follicular fluid (hFF) and correlate it with resulting embryo quality.
  • To identify potential biomarkers in hFF for predicting oocyte developmental potential.
  • To explore novel diagnostic tools for oocyte quality control in fertility treatments.

Main Methods:

  • Collected 110 hFFs from 50 oocyte donors undergoing IVF.
  • Utilized Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS) on a TripleTOF 5600+ mass spectrometer.
  • Identified 2182 proteins, with 484 quantified, and compared hFF composition with embryo quality (44 high, 39 medium, 27 low).

Main Results:

  • Identified significant variations in protein concentrations within hFFs from the same patient and between different patients.
  • Found immunoglobulin heavy constant alpha 1 (IgA1hc) at higher concentrations in hFFs associated with poor-quality embryos.
  • Observed dickkopf-related protein 3 at lower concentrations in hFFs linked to higher-quality embryos.

Conclusions:

  • Immunoglobulin heavy constant alpha 1 (IgA1hc) and dickkopf-related protein 3 are potential biomarkers for oocyte quality assessment.
  • These proteins could form the basis for a diagnostic tool for pre-fertilization screening.
  • This is particularly relevant for ethical or legal restrictions on embryo creation and selection.