Comprehensive Transcriptome Analysis Expands lncRNA Functional Profiles in Breast Cancer
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.This study identifies novel long non-coding RNAs (lncRNAs) in breast cancer, revealing their crucial roles in gene regulation and patient prognosis. These findings offer new insights into breast cancer heterogeneity and potential therapeutic targets.
Area Of Science
- Genomics
- Molecular Biology
- Cancer Research
Background
- Breast cancer is a complex, heterogeneous disease with variable patient outcomes despite similar clinical staging.
- Molecular genetic alterations significantly influence prognosis and treatment response.
- Long non-coding RNAs (lncRNAs) are key regulators of gene expression and cellular processes, vital for breast cancer subtyping.
Purpose Of The Study
- To develop a framework for identifying novel lncRNAs in breast cancer.
- To investigate the biological functions, regulatory mechanisms, and clinical relevance of these newly identified lncRNAs.
- To explore the association of lncRNA expression with breast cancer subtypes, immune response, and progression.
Main Methods
- Development of a modular framework for lncRNA identification.
- Application of the framework to a breast cancer cohort to discover novel lncRNAs.
- Analysis of genomic and chromatin features, associated genes, and regulatory networks.
- Correlation of lncRNA expression patterns with PAM50 subtyping, immune response, and immune checkpoint genes.
Main Results
- Identification of previously unannotated lncRNAs in breast cancer.
- Elucidation of the regulatory roles of novel lncRNAs in gene expression and breast cancer networks.
- Demonstration of links between lncRNA expression and hormone response, immune infiltration, and cancer progression.
- Association of lncRNA expression with immune checkpoint genes.
Conclusions
- Novel and annotated lncRNAs are integral to breast cancer biology, influencing gene expression and regulatory networks.
- These lncRNAs are associated with key aspects of breast cancer, including hormone response and immune modulation.
- The comprehensive analysis provides deeper understanding of breast cancer heterogeneity and suggests potential therapeutic strategies.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...