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Concanavalin-A-Induced Acute Liver Injury in Mice.

Tsukasa Nabekura1,2,3, Soichi Matsuo4,5,6, Akira Shibuya1,2,4

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Current Protocols
|August 10, 2024
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Summary

Acute liver injury, modeled by concanavalin A (ConA) injection in mice, involves T cell activation and myeloid cell infiltration. This study details protocols for inducing and evaluating this immune-mediated liver damage.

Keywords:
Concanavalin AT cellsacute liver injurymyeloid cellsnon‐hematopoietic cells

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Area of Science:

  • Immunology
  • Hepatology
  • Toxicology

Background:

  • Acute liver injury (ALI) is a critical condition where immune responses play a significant role, but underlying mechanisms remain unclear.
  • Concanavalin A (ConA) injection in mice serves as a standard model for studying immune-mediated ALI, mimicking aspects of autoimmune hepatitis.
  • ConA induces T cell activation and cytokine release, leading to myeloid cell infiltration and amplified liver inflammation.

Purpose of the Study:

  • To provide standardized protocols for inducing ConA-induced ALI in mice.
  • To detail methods for evaluating liver injury severity and analyzing immune and non-hematopoietic cells within the liver.
  • To offer a comprehensive guide for researchers investigating ALI pathogenesis.

Main Methods:

  • Induction of ALI via intravenous ConA administration in mice.
  • Assessment of inflammatory cytokines in plasma using quantitative reverse transcription polymerase chain reaction (qRT-PCR).
  • Histological analysis of liver sections, preparation of liver cells (immune, hepatocytes, endothelial, stellate), and flow cytometry for cell profiling and sorting.

Main Results:

  • Established protocols for reproducible ConA-induced ALI in mice.
  • Detailed methods for comprehensive analysis of cellular and molecular changes in the liver.
  • Demonstrated the influence of experimental conditions on ALI severity and immune cell infiltration.

Conclusions:

  • ConA-induced ALI is a valuable model for studying immune-mediated liver injury.
  • Standardized protocols are crucial for consistent and reliable experimental outcomes.
  • The provided methods enable detailed characterization of cellular players in ALI pathogenesis.