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Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

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Published on: April 2, 2020

Generation of Meiotic Mouse Models Using CRISPR/Cas9 Technology.

Manuel Sánchez-Martín¹, Fernando Sánchez-Sáez², Elena Llano^2,3

¹Departamento de Medicina, Universidad de Salamanca, Salamanca, Spain. adolsan@usal.es.

Methods in Molecular Biology (Clifton, N.J.)

|August 10, 2024

Summary

CRISPR/Cas9 genome editing enables precise genetic modifications in mice, advancing the study of meiosis. This protocol details creating novel mouse models with targeted gene alterations for exploring meiotic gene function.

Keywords:

CRISPR/Cas9 Genome editing Meiosis Mouse gametogenesis

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Last Updated: Jun 17, 2025

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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

Area of Science:

Genetics and Genomics
Developmental Biology
Molecular Biology

Background:

Targeted genome editing is crucial for creating animal models to study biological processes.
Meiosis in mice is a significant area of research, requiring advanced genetic tools.
CRISPR/Cas9 technology offers precision for generating specific genetic alterations.

Purpose of the Study:

To present a state-of-the-art protocol for creating mouse models with targeted meiotic gene modifications using CRISPR/Cas9.
To provide examples of specific genetic modifications in meiotic genes for research reference.

Main Methods:

Utilizing CRISPR/Cas9 genome editing technology.
Generating mice with specific point and null mutations in key meiotic genes.
Developing and validating novel mouse models for meiosis research.

Main Results:

Successful creation of novel mouse models with tailored genetic alterations in meiotic genes.
Demonstration of two distinct genetic modifications relevant to meiosis research.
Establishment of valuable reference models for studying mammalian meiosis.

Conclusions:

CRISPR/Cas9 is a powerful tool for generating precise mouse models to investigate meiotic gene function.
The presented models and protocol facilitate deeper understanding of the molecular mechanisms of meiosis.
These resources will aid researchers in elucidating the complexities of mammalian meiosis.