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Temporal Analysis of DSB Repair Outcome in Caenorhabditis elegans Meiosis
Tara Hicks1, Nicola Silva2, Sarit Smolikove3
1Department of Biology, The University of Iowa, Iowa City, IA, USA.
Abstract:
The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.
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