Related Experiment Video
Updated: Jun 17, 2025

Fruit Volatile Analysis Using an Electronic Nose
Published on: March 30, 2012
Raman spectroscopy as a tool for characterisation of quality parameters in Norwegian grown apples during ripening
Olga Monago-Maraña1, Jens Petter Wold2, Siv Fagertun Remberg3
1Department of Analytical Sciences, Faculty of Science, Universidad Nacional de Educación a Distancia (UNED), Avda. Esparta s/n, Crta. de Las Rozas-Madrid, 28232, Las Rozas, Madrid, Spain; Nofima AS - Norwegian Institute of Food, Fisheries and Aquaculture Research, Muninbakken 9-13, Breivika, Postboks 6122 Langnes, NO-9291 Tromsø, Norway.
Abstract:
This study shows for the first time the feasibility of Raman spectroscopy as a non-destructive method to follow the ripening process of apple fruits. Two different varieties of apples were studied: 'Aroma' and 'Elstar'. By visual inspection, Raman spectra showed that the starch content was higher in 'Elstar' apples compared to 'Aroma'. The degradation of starch over time could be detected in the Raman spectra, indicating that the method can be used to monitor the ripening process. The ripeness markers starch index, soluble solids content (SSC), and the sugars glucose, fructose and sucrose were determined with traditional destructive methods. Cross validated calibration models based on Raman spectroscopy were obtained for all quality parameters, and test set validation offered good results, with R2 in the range 0.4-0.86 for 'Aroma' and 0.4-0.95 for 'Elstar', respectively. The regression coefficients showed that the calibrations relied on Raman bands associated with starch and different sugars. The results suggest that Raman spectroscopy in the future could be used to determine the optimal time of harvesting and to sort apples into different degrees of ripeness.
More Related Videos
10:25Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
Published on: June 28, 2016
05:29Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
Related Concept Videos
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
NMR Spectrometers: Resolution and Error Correction
¹H NMR of Labile Protons: Temporal Resolution
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...