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Development and validation of a method to generate phenol red thread tests.

Bryan Puentes1, Erin A Hisey1, Michelle Ferneding1

  • 1Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, 95616, USA.

The Ocular Surface
|August 10, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a phenol red thread test (PRTT) as a reproducible diagnostic tool for dry eye disease. This new PRTT performs comparably to commercial versions, offering a reliable alternative when needed.

Keywords:
Awat2PRTTZone-Quickaqueous tear productiondry eye disease diagnostic test

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Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Diagnostic Test Development

Background:

  • Commercial phenol red thread tests (PRTT) are crucial for diagnosing dry eye disease.
  • Limited availability of commercial PRTT necessitates the development of alternative methods.
  • Accurate tear production measurement is vital for dry eye assessment.

Purpose of the Study:

  • To develop and validate a reproducible method for generating phenol red thread tests (PRTT).
  • To establish a cost-effective and accessible alternative to commercial PRTT.
  • To confirm the diagnostic utility of the developed PRTT in a dry eye model.

Main Methods:

  • Phenol red dye was used to impregnate cotton threads for PRTT creation.
  • In vitro wicking ability was assessed across various pH levels and time intervals.
  • In vivo diagnostic utility was evaluated in wildtype and evaporative dry eye (Awat2 KO) mice.

Main Results:

  • Two batches of self-made PRTT demonstrated comparable appearance and function to commercial tests.
  • In vitro wicking kinetics showed no significant differences between batches and commercial tests.
  • In vivo studies confirmed similar tear volume measurements and detected increased tear volume in Awat2 KO mice.

Conclusions:

  • The developed method provides a reproducible and valid diagnostic test for dry eye.
  • The self-generated PRTT serves as a reliable replacement for commercial tests.
  • This study validates a new approach for accessible dry eye diagnostics.