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Updated: Jun 17, 2025
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
Published on: June 30, 2023
Development of an amperometric biosensor for the detection of Bacillus anthracis spores
Mesut Ortatatli1, Ebru Saatci2, Zahir Kilic1
1Department of Medical CBRN Defense, University of Health Sciences, 06018, Ankara Türkiye.
Introduction:
Due to most likely use of Bacillus anthracis in biological terrorism agents, the rapid and sensitive detection of its spores is crucial in both taking prophylactic measures and proper treatment. This study aimed to develop an amperometric electrochemical immunosensor for the detection of B. anthracis spores.
Methods:
A new amperometric biosensor was designed using a combination of magnetic beads and multiplex screen-printed electrodes. This method measures changes in current intensity resulting from oxidation and reduction in the working electrode directly to spore concentrations.
Results:
A standard curve was formed to test the number of live spores between 2 × 102-2 × 104 spores/ml concentrations. LOD and LOQ values were found to be 92 and 272 spores/ml, respectively. No cross-reactions were seen for Bacillus subtilis, Bacillus cereus and Bacillus thuringiencis spores.
Conclusions:
It is shown that the designed Anthrax immunosensor has high sensitivity and selectivity with rapid detection results.
