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Updated: Jun 17, 2025
Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
Published on: January 31, 2025
Plasma Olink Proteomics Reveals Novel Biomarkers for Prediction and Diagnosis in Dilated Cardiomyopathy with Heart
Shuai Xu1,2, Ge Zhang3,4,5, Xin Tan1,2
1Department of Cardiology, The Fourth Affiliated Hospital of Soochow University, Suzhou Dushu Lake Hospital, Medical Center of Soochow University,Suzhou 215000, China.
Insights
This study identified five key proteins (SPP1, IGFBP7, F11R, CHI3L1, Plaur) as potential biomarkers for dilated cardiomyopathy with heart failure (DCM-HF), showing increased levels in patients and predictive value.
Area of Science:
- Cardiovascular Biology
- Proteomics
- Biomarker Discovery
Background:
- Dilated cardiomyopathy with heart failure (DCM-HF) poses a significant health burden.
- Identifying reliable diagnostic and prognostic biomarkers for DCM-HF is crucial.
Purpose of the Study:
- To identify and validate novel protein biomarkers for DCM-HF.
- To assess the diagnostic and predictive potential of these biomarkers.
Main Methods:
- Olink Cardiovascular III panel used for high-throughput protein expression profiling.
- ELISA validation of top candidate proteins in an independent patient cohort.
- Gene Ontology (GO) and KEGG pathway analysis to understand biological associations.
- Correlation analysis with clinical indicators and assessment of diagnostic value using AUC in public datasets.
Main Results:
- Five proteins (SPP1, IGFBP7, F11R, CHI3L1, Plaur) were significantly elevated in DCM-HF patients compared to controls.
- These proteins, along with others, are associated with DCM-HF through GO and KEGG analysis.
- Strong correlations were observed between these proteins and clinical indicators like LVEF and NT-pro BNP.
- The five core proteins demonstrated significant diagnostic value (AUCs of 0.959, 0.773, 0.803) in independent datasets.
Conclusions:
- SPP1, IGFBP7, F11R, CHI3L1, and Plaur show promise as biomarkers for DCM-HF diagnosis and prediction.
- These proteins may offer insights into DCM-HF pathogenesis.
- Further research is warranted to explore their therapeutic potential.
Abstract:
In this study, we utilized the Olink Cardiovascular III panel to compare the expression levels of 92 cardiovascular-related proteins between patients with dilated cardiomyopathy combined with heart failure (DCM-HF) (n = 20) and healthy normal people (Normal) (n = 18). The top five most significant proteins, including SPP1, IGFBP7, F11R, CHI3L1, and Plaur, were selected by Olink proteomics. These proteins were further validated using ELISA in plasma samples collected from an additional cohort. ELISA validation confirmed significant increases in SPP1, IGFBP7, F11R, CHI3L1, and Plaur in DCM-HF patients compared to healthy controls. GO and KEGG analysis indicated that NT-pro BNP, SPP1, IGFBP7, F11R, CHI3L1, Plaur, BLM hydrolase, CSTB, Gal-4, CCL15, CDH5, SR-PSOX, and CCL2 were associated with DCM-HF. Correlation analysis revealed that these 13 differentially expressed proteins have strong correlations with clinical indicators such as LVEF and NT-pro BNP, etc. Additionally, in the GEO-DCM data sets, the combined diagnostic value of these five core proteins AUC values of 0.959, 0.773, and 0.803, respectively indicating the predictive value of the five core proteins for DCM-HF. Our findings suggest that these proteins may be useful biomarkers for the diagnosis and prediction of DCM-HF, and further research is prompted to explore their potential as therapeutic targets.
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