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Related Concept Videos

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Related Experiment Video

Updated: Jun 17, 2025

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
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Genome Wide Conditional Mouse Knockout Resources.

C Kaloff1, K Anastassiadis2, A Ayadi3

  • 1Institute of Developmental Genetics, Helmholtz Zentrum Muenchen, D-85764 Neuherberg, Germany.

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|August 12, 2024
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Summary
This summary is machine-generated.

The International Knockout Mouse Consortium created over 18,500 gene mutations in mouse embryonic stem cells, resulting in thousands of mutant mouse strains and cre-driver lines for research. These resources are available at www.mousephenotype.org.

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Area of Science:

  • * Genetics and Genomics
  • * Mammalian Genetics
  • * Mouse Models

Background:

  • * The International Knockout Mouse Consortium (IKMC) established high-throughput pipelines for gene trapping and targeting.
  • * This initiative generated conditional mutations for over 18,500 genes in C57BL/6N mouse embryonic stem (ES) cells.
  • * These ES cell lines are archived and available as a frozen resource for the research community.

Purpose of the Study:

  • * To create and provide access to a comprehensive library of mouse models for genetic research.
  • * To facilitate the analysis of gene-disease associations through standardized mouse strains.
  • * To support biomedical research by offering a diverse collection of genetic tools.

Main Methods:

  • * High-throughput gene trapping and gene targeting in mouse ES cells.
  • * Production of conditional mutations for a large number of genes.
  • * Development of inducible cre-driver mouse strains and cre-driver rAAVs.

Main Results:

  • * Over 18,500 genes have conditional mutations in archived ES cells.
  • * More than 6,000 mutant mouse strains have been generated by IKMC and the International Mouse Phenotyping Consortium (IMPC).
  • * A resource of 250 inducible cre-driver mouse strains and 27 cre-driver rAAVs was established.

Conclusions:

  • * The IKMC/IMPC resource provides a standardized library of mouse models with defined genetic backgrounds.
  • * These resources enable the analysis of gene-disease associations in diverse genetic contexts.
  • * The IKMC/IMPC resource is expected to significantly impact biomedical research.