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[Morphological research on DMSO-perfused kidneys]
Abstract:
It is reported on the morphological investigations of kidneys which had been perfused with the cryoprotective dimethylsulfoxide (DMSO). Some of these kidneys were stored for 72 hours at -4.5 degrees C and then retransplanted. The light-microscopic and electron-optical findings show considerable changes of the cells of the proximal tubulus epithelium and the collecting tubules. These in most cases focal findings of different extension do not differ on principle from the results in preservation techniques without DMSO. The dilatation of the basal labyrinth of the tubulus cell as well as the fissuration and vacuolization in the cytoplasm give references to a passive transport of substance by the cell. The results of the DMSO-perfused and at -4.5 degrees C stored kidneys show that for a beginning of the function an optimal perfusion must be supposed. On the one hand a sufficient concentration of freezing defense remedies in the renal tissue is necessary, on the other hand an optimum removal of the DMSO in the reperfusion is necessary, in order to keep the osmotic load of the kidney as low as possible.
Insights
Dimethyl sulfoxide (DMSO) cryoprotection of kidneys causes cell changes, similar to non-DMSO methods. Optimal kidney function after storage requires sufficient cryoprotectant and efficient DMSO removal during reperfusion.
Area of Science:
- Nephrology
- Transplantation immunology
- Cell biology
Context:
- Kidney preservation for transplantation is crucial.
- Cryoprotective agents like dimethyl sulfoxide (DMSO) are used.
- Understanding cellular effects of cryopreservation is vital.
Purpose:
- To investigate the morphological changes in kidneys preserved with DMSO.
- To evaluate the impact of cold storage and DMSO on kidney tissue.
- To correlate morphological findings with potential functional recovery.
Summary:
- Kidneys perfused with dimethyl sulfoxide (DMSO) and stored at -4.5°C for 72 hours were examined.
- Light and electron microscopy revealed significant changes in proximal tubule and collecting tubule cells.
- These DMSO-induced changes, including basal labyrinth dilatation and cytoplasmic vacuolization, suggest passive substance transport.
Impact:
- DMSO cryopreservation results in cellular alterations comparable to non-DMSO methods.
- Successful kidney retransplantation and function depend on optimal perfusion and reperfusion strategies.
- Efficient DMSO removal during reperfusion is critical to minimize osmotic stress and support kidney function recovery.