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[Kidney function and bacteriological and histological research in the therapy of experimental E. coli pyelonephritis
Abstract:
40 uninephrectomized male Wistar rats with an experimental E.-coli-022-pyelonephritis (PN) were treated twice daily for 9 days with 30 mg trimethoprim and 150 mg sulfamethoxazole (TMP-SMO) i.p. Bacteriologically most of the kidneys became sterile. Histologically a significant reduction of the frequency of severe PN was found in the treated group. The biologic half-life of 131I-hippuran indicated a decrease of excretory function which was reversible. Urine osmolality and osmotic clearance were increased after oral water loading in 10 untreated control animals with PN but not in the treated group. The 9 day treatment had a favourable effect bacteriologically, histologically and also on renal function.
Insights
Trimethoprim-sulfamethoxazole treatment effectively cleared E. coli pyelonephritis in rats, improving kidney health and function. The positive effects on renal function were reversible after the 9-day antibiotic course.
Area of Science:
- Nephrology
- Pharmacology
- Microbiology
Background:
- Pyelonephritis (PN) is a significant kidney infection.
- Experimental E. coli O22 pyelonephritis model in rats is used to study treatment efficacy.
- Assessing the impact of antibiotics on renal function is crucial.
Purpose of the Study:
- To evaluate the efficacy of trimethoprim-sulfamethoxazole (TMP-SMO) in treating experimental E. coli pyelonephritis in rats.
- To assess the impact of TMP-SMO treatment on kidney histology and function.
Main Methods:
- 40 male Wistar rats with induced E. coli pyelonephritis were treated with TMP-SMO (30 mg/150 mg) twice daily for 9 days.
- Kidney bacterial cultures, histological examination, and renal function tests (131I-hippuran half-life, urine osmolality, osmotic clearance) were performed.
Main Results:
- Bacteriological analysis showed most treated kidneys became sterile.
- Histological examination revealed a significant reduction in severe pyelonephritis.
- Renal excretory function, assessed by 131I-hippuran biologic half-life, showed reversible decreases.
- Oral water loading tests indicated preserved urine concentrating ability in treated rats compared to controls.
Conclusions:
- A 9-day course of trimethoprim-sulfamethoxazole demonstrated favorable bacteriologic and histologic effects in experimental pyelonephritis.
- The treatment also positively impacted renal function, with observed effects being reversible.
- TMP-SMO is an effective therapeutic option for E. coli pyelonephritis, with implications for preserving kidney function.