Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

88.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AAV vector production in suspension cells using PEI transfection and sodium butyrate with orthogonal assessment of function and quality.

Molecular therapy. Advances·2026
Same author

Risk-adapted HLA delisting and imlifidase-enabled deceased-donor kidney transplantation in highly sensitized kidney transplant candidates: a German expert consensus report.

Frontiers in immunology·2026
Same author

PD-L1 Expression in Mesenchymal Stem/Stromal Cells: Impacts on Innate and Adaptive Immunity, Therapeutic Potential, and Biomarker Utility.

International journal of molecular sciences·2026
Same author

A roadmap for supporting the development of advanced therapy medicinal products in a European framework.

The Lancet regional health. Europe·2026
Same author

Preformed donor-specific antibodies are associated with acute rejection and biliary complications after liver transplantation: A Swiss Transplant Cohort Study analysis.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·2026
Same author

Association of Torque Teno Virus DNA Load and Tacrolimus in Peripheral Blood Mononuclear Cells of Adult Kidney Transplant Patients.

Therapeutic drug monitoring·2026

Related Experiment Video

Updated: Jun 17, 2025

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

38.9K

High resolution HLA genotyping with third generation sequencing technology-A multicentre study.

Stéphane Buhler1, Maja Nørgaard2, Rudi Steffensen3

  • 1Transplantation Immunology Unit and National Reference Laboratory for Histocompatibility, Department of Diagnostic, Geneva University Hospitals, Geneva, Switzerland.

HLA
|August 12, 2024
PubMed
Summary

Nanopore sequencing offers a fast and practical method for high-resolution human leukocyte antigen (HLA) typing, showing high concordance with next-generation sequencing (NGS). This technique is valuable for diagnostics and research, despite some data handling challenges.

Keywords:
HLA‐typingNanoTYPEdeceased donor typingdonor specific antibody determinationhigh‐resolution typingnanopore sequencingsingle sample HLA‐typingthird generation sequencing

More Related Videos

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

11.6K
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.1K

Related Experiment Videos

Last Updated: Jun 17, 2025

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

38.9K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

11.6K
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.1K

Area of Science:

  • Genomics
  • Molecular Biology
  • Immunogenetics

Background:

  • Current molecular HLA typing methods are evolving rapidly.
  • Next-generation sequencing (NGS) is crucial for high-resolution HLA typing, essential for stem cell transplantation and disease association studies.
  • Real-time PCR is standard for solid organ allocation, but not yet ideal for deceased donors.

Purpose of the Study:

  • To evaluate the concordance and practicability of nanopore sequencing (TGS) for high-resolution HLA typing in a multicentre clinical setting.
  • To assess the performance of the NanoTYPE™ kit and NanoTYPER™ software for HLA typing.
  • To compare nanopore sequencing results with established NGS methods.

Main Methods:

  • A multicentre clinical study involving 381 samples.
  • Utilized nanopore sequencing (TGS) with the NanoTYPE™ kit and NanoTYPER™ software.
  • Analyzed concordance across 11 HLA loci (HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1, -DPB1).

Main Results:

  • Achieved 99.58% concordance for 11 HLA loci when comparing nanopore sequencing to NGS.
  • High-quality sequencing was demonstrated, with over 97% of quality control (QC) values passing.
  • Identified that 'inspect' or 'failed' QC warnings do not necessarily indicate incorrect HLA typing.

Conclusions:

  • Nanopore sequencing is a fast, flexible, and easily implementable method for high-resolution HLA typing in diagnostic laboratories.
  • While challenges like exon coverage and data handling exist, nanopore sequencing shows significant potential for clinical applications and basic research in epigenetics and genomics.