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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Jun 17, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Implementing distinct spatial proteogenomic technologies: opportunities, challenges, and key considerations.

Bram Verstappe^1,2, Charlotte L Scott^1,2,3

¹Laboratory of Myeloid Cell Biology in Tissue Damage and Inflammation, VIB-UGent Center for Inflammation Research, Ghent, Belgium.

Clinical and Experimental Immunology

|August 12, 2024

Summary

This summary is machine-generated.

Proteogenomic technologies, including spatial analyses, are revolutionizing tissue complexity understanding. This review guides experimental design for generating comprehensive human atlases and identifying disease biomarkers.

Keywords:

cell activation cellular immunology inflammation molecular biology

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Published on: May 22, 2018

Area of Science:

Proteogenomics
Spatial biology
Biomarker discovery

Background:

Recent advances in proteogenomic technologies have significantly enhanced our understanding of cellular complexity in tissues.
Initiatives like the Human Cell Atlas aim to profile all human cells, paving the way for disease biomarker and therapeutic target identification.

Purpose of the Study:

To highlight key considerations for experimental design and analysis in proteogenomic studies.
To assist researchers in navigating the rapidly evolving technological landscape for generating tissue atlases.

Main Methods:

Review of current proteogenomic technologies, emphasizing spatial analysis capabilities.
Discussion of factors influencing the selection of optimal techniques for specific biological questions.

Main Results:

Identification of critical considerations for experimental design in proteogenomics.
Guidance on selecting appropriate technologies for tissue profiling and atlas generation.

Conclusions:

Careful experimental design is crucial for successful proteogenomic studies and the development of human tissue atlases.
Navigating technological advancements is key to unlocking the potential of proteogenomics for biomarker and therapeutic discovery.