Related Experiment Video

Updated: Aug 12, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

Radioiodination of a photoactivatable heterobifunctional reagent

I Ji, J Shin, T H Ji

    Analytical Biochemistry
    |December 1, 1985
    PubMed

    More Related Videos

    An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group07:23

    An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

    Published on: October 10, 2016

    Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets10:16

    Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

    Published on: March 12, 2019

    Related Concept Videos

    Regioselectivity and Stereochemistry of Hydroboration02:36

    Regioselectivity and Stereochemistry of Hydroboration

    A significant aspect of hydroboration–oxidation is the regio- and stereochemical outcome of the reaction.
    Hydroboration proceeds in a concerted fashion with the attack of borane on the π bond, giving a cyclic four-centered transition state. The –BH2 group is bonded to the less substituted carbon and –H to the more substituted carbon. The concerted nature requires the simultaneous addition of –H and –BH2 across the same face of the alkene giving syn stereochemistry.
    Photochemical Electrocyclic Reactions: Stereochemistry01:26

    Photochemical Electrocyclic Reactions: Stereochemistry

    The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
    Selection Rules: Photochemical Activation
    Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene01:13

    Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene

    Bromination and chlorination of aromatic rings by electrophilic aromatic substitution reactions are easily achieved, but fluorination and iodination are difficult to achieve. Fluorine is so reactive that its reaction with benzene is difficult to control, resulting in poor yields of monofluoroaromatic products. To address this, Selectfluor reagent is used as a fluorine source in which a fluorine atom is bonded to a positively charged nitrogen.
    Cycloaddition Reactions: MO Requirements for Photochemical Activation01:12

    Cycloaddition Reactions: MO Requirements for Photochemical Activation

    Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.
    Redox Titration: Iodimetry and Iodometry01:23

    Redox Titration: Iodimetry and Iodometry

    Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...