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Mutagenic metabolites in rat urine after dermal exposure to 1,3-diaminobenzene
Abstract:
Amberlite XAD-2 purified urine extracts from rats treated dermally with 1,3-diaminobenzene were fractionated by HPLC and the metabolites were identified by GC-MS. Mutagenic activity in Salmonella typhimurium TA 98 was equally distributed between the two fractions, none of which contained the parent amine. The first fraction, eluted between 2-7 minutes, contained only a very small amount of presently unidentified metabolites, while the second fraction eluted between 14-19 minutes contained N,N'-diacetyl-1,3-diaminobenzene. Both fractions showed minor, but still significant activity even without metabolic activation. A non-mutagenic fraction, eluted between 10-12 minutes contained N-acetyl -acetyl-1,3-diaminobenzene and N,N'-diacetyl-2,4-diaminophenol.
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