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Preparation of a specific high-titer antibody against rat kidney renin
Specific high-titer antibodies to rat kidney renin were raised using pure rat kidney renin conjugated with tetanus toxoid as antigen. The titers of two antisera for 50% inhibition of rat kidney renin activity were 80 000 and 700 000, respectively. The antibody inhibited the enzyme activities of hog kidney renin and mouse submaxillary gland renin at concentrations 1 000 times higher than required for rat kidney renin but did not inhibit human renal renin or rat kidney cathepsin D. The antibody can be used for characterizing the physiological and pathophysiological role of renin in blood pressure regulation, for the histochemical localization of renin in rat tissues and for distinguishing specific renin activity from the nonspecific renin-like activity of cathepsin D.
Specific high-titer antibodies to rat kidney renin were raised using pure rat kidney renin conjugated with tetanus toxoid as antigen. The titers of two antisera for 50% inhibition of rat kidney renin activity were 80 000 and 700 000, respectively. The antibody inhibited the enzyme activities of hog kidney renin and mouse submaxillary gland renin at concentrations 1 000 times higher than required for rat kidney renin but did not inhibit human renal renin or rat kidney cathepsin D. The antibody can be used for characterizing the physiological and pathophysiological role of renin in blood pressure regulation, for the histochemical localization of renin in rat tissues and for distinguishing specific renin activity from the nonspecific renin-like activity of cathepsin D.