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Circular RNA NFIX Functions as an Oncogene in Non-Small Cell Lung Cancer by Modulating the miR-214-3p/TRIAP1 Axis

Guohua Liu1, Hanbing Shi1, Hongyan Zheng1

  • 1Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, China.

The Clinical Respiratory Journal
|August 12, 2024
PubMed
Abstract

Insights

Circular RNA NFIX (circRNA NFIX) promotes non-small cell lung cancer (NSCLC) by downregulating miRNA-214-3p and upregulating TRIAP1. Silencing circRNA NFIX inhibits NSCLC cell proliferation and induces apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Circular RNA NFIX (circRNA NFIX) is implicated as an oncogene in glioma.
  • The expression and function of circRNA NFIX in non-small cell lung cancer (NSCLC) remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression profile of circRNA NFIX in NSCLC.
  • To elucidate the functional role of circRNA NFIX in NSCLC cell proliferation and apoptosis.
  • To explore the molecular mechanism involving circRNA NFIX, miRNA-214-3p, and TRIAP1 in NSCLC.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to assess expression levels of circRNA NFIX, miRNA-214-3p, and TRIAP1.
  • Bioinformatic prediction (Starbase) and dual-luciferase reporter assays to validate interactions between circRNA NFIX/TRIAP1 and miRNA-214-3p.
  • MTT assays and flow cytometry to evaluate cell proliferation and apoptosis, respectively.
  • Western blot analysis to measure apoptosis-related protein expression.

Main Results:

  • circRNA NFIX was found to be upregulated, while miRNA-214-3p was downregulated in NSCLC tissues and cell lines.
  • Suppression of circRNA NFIX inhibited NSCLC cell proliferation and induced apoptosis, correlating with increased miRNA-214-3p expression.
  • TRIAP1 was identified as a target of miRNA-214-3p, with its expression negatively regulated by miRNA-214-3p. Overexpression of TRIAP1 reversed the effects of miRNA-214-3p mimic on NSCLC cells.

Conclusions:

  • circRNA NFIX silencing inhibits NSCLC cell proliferation and induces apoptosis via the circRNA NFIX/miRNA-214-3p/TRIAP1 axis.
  • This axis represents a potential diagnostic and therapeutic target for NSCLC.
Keywords:
NSCLCTRIAP1circRNA NFIXmiRNA‐214‐3p

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