Antifungal Patterns of Dermatophytes: A Pathway to Antifungal Stewardship in Eastern India
Satyendra P Yadav1, Manoj Kumar2, Kumari Seema2
1Microbiology, Phulo Jhano Medical College and Hospital, Dumka, IND.
Abstract:
Background Dermatophytosis is a superficial fungal infection caused by a group of pathogenic keratinophilic fungi. The increase in the incidence of superficial fungal infections, combined with the emergence of antifungal resistance, represents both a global health challenge and a considerable economic burden. Recent years have witnessed a surge in dermatophytosis cases, accompanied by the emergence of antifungal-resistant strains. This study aimed to analyze the in vitro antifungal susceptibility patterns and determine the minimum inhibitory concentrations (MIC) of antifungal drugs among isolated species using the broth microdilution method. Methodology This cross-sectional study was conducted between September 2021 and August 2022. Patients with symptoms or clinical features of fungal infection, including skin, hair, and nail lesions indicative of Tinea infections, were included. Samples underwent processing, including potassium hydroxide (KOH) mounting, direct microscopic examination, and culture on Sabouraud Dextrose Agar (SDA) with antibiotics. Antifungal susceptibility testing was subsequently conducted. Results Trichophyton mentagrophytes emerged as the most common isolate among patients with Tinea infections. MIC values of various drugs were analyzed, with itraconazole exhibiting a minimum MIC of 0.03 µg/ml and a maximum of 0.50 µg/ml. Terbinafine showed an MIC of 0.010 µg/ml and a maximum of 1.00 µg/ml. Ketoconazole had a minimum MIC of 0.03 µg/ml and a maximum of 0.50 µg/ml. Fluconazole exhibited a minimum MIC of 0.10 µg/ml and a maximum of 1.00 µg/ml. Lastly, miconazole demonstrated a minimum MIC of 0.03 µg/ml and a maximum of 2.00 µg/ml. Conclusion Accurate diagnosis is crucial for fungal infections to enable early treatment and reduce transmission. With an increasing trend in resistance among dermatophytes, there is a growing need to conduct susceptibility testing of antifungal agents, particularly in cases of long-term infections, recurrent infections, and individuals who do not respond to medication.
Insights
Dermatophytosis, a fungal infection, is rising globally. This study found Trichophyton mentagrophytes most common and analyzed antifungal drug effectiveness, highlighting the need for susceptibility testing due to increasing resistance.
Area of Science:
- Medical Mycology
- Antimicrobial Resistance
- Dermatology
Background:
- Dermatophytosis, a superficial fungal infection, is a growing global health concern due to increasing incidence and antifungal resistance.
- Emergence of antifungal-resistant strains poses a significant challenge in treating common fungal infections of the skin, hair, and nails.
Purpose of the Study:
- To investigate the in vitro antifungal susceptibility patterns of fungal isolates from Tinea infections.
- To determine the minimum inhibitory concentrations (MIC) of various antifungal drugs against common dermatophyte species.
Main Methods:
- Cross-sectional study conducted from September 2021 to August 2022.
- Inclusion of patients with clinical features of Tinea infections.
- Broth microdilution method used for antifungal susceptibility testing and MIC determination.
Main Results:
- Trichophyton mentagrophytes was identified as the most prevalent isolate.
- Minimum Inhibitory Concentration (MIC) ranges were determined for itraconazole, terbinafine, ketoconazole, fluconazole, and miconazole.
- Varied MIC values indicated differential susceptibility of isolates to the tested antifungal agents.
Conclusions:
- Accurate diagnosis and early treatment are essential to reduce transmission and manage fungal infections effectively.
- Increasing antifungal resistance necessitates routine susceptibility testing, especially for persistent, recurrent, or treatment-unresponsive cases.
