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Effects of antigens on mononuclear leukocyte chemiluminescence
Abstract:
Influenza virus and Candida albicans allergenic extract elicit alterations in the oxidative metabolic activity of human mononuclear leukocytes (MNL). MNL preparations (greater than 96% lymphocytes, less than 4% monocytes) exposed in vitro to inactivated influenza virus displayed significantly enhanced oxidative activity, as demonstrated by luminol-amplified chemiluminescence (CL). MNL from donors who had received influenza vaccine and unimmunized donors showed similar levels of virus-stimulated CL. In contrast, MNL exposed to Candida (a macrophage dependent lymphocyte stimulator) displayed significant suppression in oxidative activity. Our findings support the hypothesis that oxidative activity holds an important role in the early events of the immune response.
Insights
Influenza virus exposure boosts human mononuclear leukocyte (MNL) oxidative activity, while Candida albicans suppresses it. This suggests oxidative activity is crucial for early immune responses.
Area of Science:
- Immunology
- Cellular Biology
- Biochemistry
Background:
- Human mononuclear leukocytes (MNL) play a key role in immune responses.
- Oxidative metabolic activity is a critical component of cellular function during immune interactions.
Purpose of the Study:
- To investigate the impact of influenza virus and Candida albicans on the oxidative metabolic activity of MNL.
- To explore the role of oxidative activity in the early stages of the immune response.
Main Methods:
- In vitro exposure of MNL preparations (primarily lymphocytes) to inactivated influenza virus and Candida albicans allergenic extract.
- Measurement of oxidative activity using luminol-amplified chemiluminescence (CL).
Main Results:
- Influenza virus stimulation led to significantly enhanced oxidative activity in MNL.
- Candida albicans exposure resulted in a significant suppression of MNL oxidative activity.
- No significant difference in virus-stimulated CL was observed between vaccinated and unvaccinated donors.
Conclusions:
- Oxidative metabolic activity is modulated differently by various immune stimuli.
- Findings support the hypothesis that oxidative activity is integral to the early events of the immune response.