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Mycobolome of Phialomyces Macrosporus Across OSMAC-Based Assorted Culture Media
Vitória Evelyn Teles de Jesus1, Yasmin Alvarenga1, Elisangela F Boffo1
1Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115, Brasil.
Abstract:
The fungus Phialomyces macrosporus was cultured using the One Strain Many Compounds (OSMAC) strategies to evaluate its metabolome. Variations in the nutrient culture media, culture regime, and cultivation parameters can significantly influence fungal extract quantity and chemical diversity. This study aimed to explore the mycobolome of P. macrosporus in five different culture media and two different cultivation conditions using NMR-based metabolomics. Principal component analysis (PCA) of 1H-NMR spectra revealed clear differentiation between these samples, highlighting the rice dextrose agar medium (RDA) and potato dextrose broth (PDB) as standard complex media for conducting a fungal metabolite screening program.
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