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Updated: Jun 17, 2025
08:30
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
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Detecting serologically difficult ABO blood groups using single-molecule real-time sequencing technology.
Zhe Wang1, Yushuang Chu1, Yanlin Xiao1
1Department of Blood Transfusion, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Vox Sanguinis
|August 13, 2024
Summary
Third-generation long-read sequencing accurately determines complete ABO gene sequences, identifies rare subtypes, and distinguishes haplotypes. This advanced method aids in resolving complex blood group discrepancies.
Area of Science:
- Genetics
- Molecular Biology
- Immunology
Background:
- Third-generation long-read sequencing is emerging for blood group system analysis.
- This technology offers long read lengths and single-molecule sequencing capabilities.
- It enables complete gene sequencing and haplotype distinction.
Purpose of the Study:
- To analyze ABO gene sequences, including flanking regions, in serologically difficult blood samples.
- To evaluate the utility of long-read sequencing for complex ABO blood group typing.
Main Methods:
- Screened 21 patients with ABO typing discrepancies.
- Utilized serological testing, preliminary ABO genotyping (PCR-SSP), and single-molecule real-time (SMRT) sequencing.
- Validated results using PCR sequence-based typing (PCR-SBT).
Main Results:
- Identified 15 common and 6 rare ABO subtypes.
- Discovered a novel ABO allele (ABO*B.NEW) and an allelic recombination event.
- Obtained 42 haplotype sequences using SMRT sequencing, confirming exon sequences with PCR-SBT.
Conclusions:
- Single-molecule real-time (SMRT) sequencing accurately provides complete ABO gene sequences.
- SMRT sequencing effectively distinguishes haplotypes and identifies allelic recombination.
- This technology is valuable for resolving complex blood group genetics.
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