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Lipid production from corn straw by cellobiohydrolase and delta-6 desaturase engineered Mucor circinelloides strains
Yao Zhang1,2, Zhuo Liu3, Yan Sun3
1Food Bioengineering and Technology Laboratory, Department of Food Science and Nutrition, College of Culture and Tourism, University of Jinan, 13 Shungeng Road, Jinan, 250022, People's Republic of China. shc_zhangy@ujn.edu.cn.
Abstract:
Previously, we constructed engineered M. circinelloides strains that can not only utilize cellulose, but also increase the yield of γ-linolenic acid (GLA). In the present study, an in-depth analysis of lipid accumulation by engineered M. circinelloides strains using corn straw was to be explored. When a two-stage temperature control strategy was adopted with adding 1.5% cellulase and 15% inoculum, the engineered strains led to increases in the lipid yield (up to 1.56 g per 100 g dry medium) and GLA yield (up to 274 mg per 100 g dry medium) of 1.8- and 2.3-fold, respectively, compared with the control strain. This study proved the engineered M. circinelloides strains, especially for Mc-C2PD6, possess advantages in using corn straw to produce GLA. This work provided a reference for transformation from agricultural cellulosic waste to functional lipid in one step, which might play a positive role in promoting the sustainable development of biological industry.
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