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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 17, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Label free tracking to quantify nanoparticle diffusion through biological media.

Genevieve Schleyer¹, Eann A Patterson², Judith M Curran³

¹Department of Materials, Design & Manufacturing Engineering, University of Liverpool, Brownlow Hill, Liverpool, UK. G.Schleyer@liverpool.ac.uk.

Scientific Reports

|August 13, 2024

Summary

This summary is machine-generated.

This study used a label-free optical tracking technique to measure gold nanoparticle diffusion in biological media. Protein concentration, charge, and diameter were found to influence nanoparticle movement, advancing nanotechnology applications.

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Last Updated: Jun 17, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

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Published on: September 5, 2019

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Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Published on: March 5, 2021

Area of Science:

Nanotechnology
Biophysics
Materials Science

Background:

Nanoparticle diffusion in biofluids is critical for developing new technologies.
Current tracking methods like fluorescence microscopy may alter nanoparticle behavior.
A need exists for label-free techniques to accurately study nanoparticle dynamics.

Purpose of the Study:

To experimentally determine the diffusion coefficient of gold nanoparticles using a label-free optical tracking method.
To evaluate how protein concentration, charge, and diameter affect nanoparticle diffusion in biological media.
To develop experimental methods for quantitative analysis of nanoparticle dynamics in biological environments.

Main Methods:

Utilized a label-free optical tracking technique to monitor gold nanoparticle diffusion.
Investigated nanoparticles with varying diameters (10-100 nm) and charges (positive/negative).
Dispersed nanoparticles in cell culture media with controlled serum protein concentrations.

Main Results:

Established experimental regimes for quantitative analysis of nanoparticle dynamics.
Demonstrated that dynamic protein interactions significantly influence nanoparticle diffusion.
Quantified the diffusion coefficient of gold nanoparticles under varying biological conditions.

Conclusions:

Label-free optical tracking provides accurate measurements of nanoparticle diffusion.
Protein interactions in biofluids play a key role in modulating nanoparticle transport.
Findings contribute to the understanding and design of nanoparticles for biological applications.