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Related Concept Videos

  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Value Of Altered Methylation Patterns Of Genes Ranbp3, Lcp2 And Grap2 In Cfdna In Breast Cancer Diagnosis.
  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • Value Of Altered Methylation Patterns Of Genes Ranbp3, Lcp2 And Grap2 In Cfdna In Breast Cancer Diagnosis.

    • Related Experiment Video

    Methyl-binding DNA capture Sequencing for Patient Tissues
    08:40

    Methyl-binding DNA capture Sequencing for Patient Tissues

    Published on: October 31, 2016

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    Value of altered methylation patterns of genes RANBP3, LCP2 and GRAP2 in cfDNA in breast cancer diagnosis.

    Qin Hu1, Yu Mao2, Haomiao Lan2

    • 1Zigong Maternal and Child Health Hospital, Department of Clinical Laboratory, Zigong, China.

    Journal of Medical Biochemistry
    |August 14, 2024

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    Methylation patterns in plasma circulating free DNA (cfDNA) for RANBP3, LCP2, and GRAP2 genes show promise for breast cancer detection. These blood-based markers closely reflect tissue methylation, aiding in supplementary breast cancer diagnosis.

    Keywords:
    LCP2 GRAP2RANBP3breast cancercfDNAmethylation pattern

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    Related Experiment Videos

    Methyl-binding DNA capture Sequencing for Patient Tissues
    08:40

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    Published on: October 31, 2016

    8.6K
    Serum and Plasma Copy Number Detection Using Real-time PCR
    09:21

    Serum and Plasma Copy Number Detection Using Real-time PCR

    Published on: December 15, 2017

    11.4K
    Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
    07:50

    Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

    Published on: September 18, 2020

    5.5K

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • Tumour methylation changes are critical in cancer development.
    • Plasma cell-free DNA (cfDNA) methylation patterns may mirror tumour-specific epigenetic alterations.
    • Identifying reliable cfDNA methylation markers is crucial for non-invasive cancer diagnostics.

    Purpose of the Study:

    • To investigate if plasma cfDNA methylation patterns reflect tumour methylation changes at specific CpG sites.
    • To evaluate the potential of RANBP3, LCP2, and GRAP2 gene methylation as biomarkers for breast cancer detection.
    • To assess the correlation between cfDNA methylation and tissue methylation in breast cancer patients.

    Main Methods:

    • Analysis of 850k whole-methylation sequencing data to identify candidate markers.
    • Methylation analysis of RANBP3, LCP2, and GRAP2 genes in breast cancer tissues and adjacent tissues from 17 patients.
    • Comparison of gene methylation levels in plasma cfDNA versus tissue samples.
    • Investigation of methylation patterns across different breast cancer molecular subtypes and pathological grades.

    Main Results:

    • RANBP3, LCP2, and GRAP2 genes showed significant methylation differences between cancer and adjacent tissues.
    • These genes demonstrated high diagnostic efficiencies in breast cancer tissues (91.0%–92.2%).
    • Plasma cfDNA methylation levels for these genes closely correlated with tissue methylation, showing diagnostic efficiencies of 77.6%–83.9%.

    Conclusions:

    • RANBP3, LCP2, and GRAP2 gene methylation in plasma cfDNA are promising biomarkers for supplementary breast cancer diagnosis.
    • This study supports the utility of cfDNA methylation analysis for early cancer detection.
    • Further research into cfDNA methylation patterns could advance early detection for various cancers.