Immunocytochemistry of peptidergic systems in the pond snail Lymnaea stagnalis

Evidence suggests that there exists in the animal kingdom a family of biologically active peptides whose members are related to the molluscan cardio-active tetrapeptide FMRFamide (Phe-Met-Arg-Phe-NH2). Immunocytochemical and ultrastructural studies indicate that several family-members occur in the pond snail Lymnaea stagnalis. Monoclonal antibodies were raised to whole brain homogenates of the pond snail. Selection of antibody producing hybridomas was carried out by staining sections of the central nervous system of the snail with the supernatants of the hybridomas. Certain antibodies stain selectively known (neuro)endocrine centres of the snail, others are directed against particular groups of neurons. It is argued that these antibodies were raised against biologically active peptides and/or their precursors. The antibodies may be used for the isolation of these peptides.