Contribution of Surface Adhesins of Lacticaseibacillus paracasei S-NB to Its Intestinal Adhesion and Colonization
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View abstract on PubMed
Summary
This summary is machine-generated.Surface adhesins on lactic acid bacteria (LAB) impact intestinal colonization. This study found that capsular polysaccharide synthesis and surface protein transport are crucial for Lacticaseibacillus paracasei S-NB adhesion and gut persistence.
Area Of Science
- Microbiology
- Gastroenterology
- Bacterial Genetics
Background
- Intestinal retention and persistence of lactic acid bacteria (LAB) are strain-specific.
- Bacterial surface components influence LAB adhesion and colonization.
- The precise role of surface adhesins in LAB intestinal colonization requires further investigation.
Purpose Of The Study
- To investigate the contribution of surface adhesins in Lacticaseibacillus paracasei S-NB to intestinal adhesion and colonization.
- To elucidate the function of specific genes (wzd, wze, srtA) involved in surface adhesin synthesis.
- To assess the impact of gene knockouts on bacterial surface properties and in vivo colonization.
Main Methods
- Generation of seven gene knockout mutants of Lacticaseibacillus paracasei S-NB using a Cre-lox-based recombination system.
- Analysis of bacterial surface properties including capsule layer thickness, hydrophobicity, and surface charge.
- In vivo colonization experiments in the ileal and colon lumen of host models.
Main Results
- Mutants S-NBΔ7576, S-NBΔdlt, and S-NBΔsrtA exhibited a thinner capsule layer compared to wild-type S-NB.
- Gene mutations (S-NB_7576, S-NB_srtA) affected surface hydrophobicity and charge, with varying impacts on adhesion.
- S-NBΔ7576 and S-NBΔsrtA mutants showed significantly reduced intestinal colonization in both ileal and colon segments.
Conclusions
- Capsular polysaccharide synthesis is vital for the adhesion and intestinal colonization of L. paracasei S-NB.
- Surface protein transport, mediated by sortase A, also plays a significant role in L. paracasei S-NB intestinal colonization.
- Understanding these surface components is key to optimizing LAB for probiotic applications.
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