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An automated method for thrombocyte counting in capillary microsamples.

Caroline Vasard Boesen¹, Vibeke Staun Christensen¹, Klaus Rosenkilde Jensen¹

¹Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.

International Journal of Laboratory Hematology

|August 14, 2024

Summary

This study presents an automated, low-volume method for thrombocyte counting in capillary blood. The new method improves analytical quality and reduces turnaround times, offering a viable alternative for both adult and pediatric samples.

Keywords:

Sysmex biochemistry blood components neonatal hematology platelets thrombocytopenia

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Area of Science:

Hematology
Clinical Chemistry
Laboratory Medicine

Background:

Developing automated, low-volume methods for thrombocyte counting in capillary blood is crucial for efficient laboratory diagnostics.
The Sysmex predilution (PD) mode offers a potential platform for such advancements.

Purpose of the Study:

To develop and evaluate an automated, low-volume method for thrombocyte counting using capillary blood with the Sysmex PD mode.
To assess the analytical quality, preanalytical factors, and turnaround time of this new method.

Main Methods:

Microsamples (50 μL blood) were prepared and resuspended in 300 μL DCL CellPack.
Thrombocyte counts were performed using impedance (PLT-I) and fluorescence (PLT-F) channels.
Imprecision, bias, and preanalytical factors (skin-piercing, storage, pneumatic tube transport) were evaluated in over 394 adult microsamples, with comparisons to pediatric samples.

Main Results:

The automated method using PLT-F showed imprecision of 1.1%-3.7% and bias of 10.1%.
Post-skin-piercing, bias was 8.1% and imprecision 1.9%. Counts remained stable after 4 hours at room temperature and after pneumatic tube transport.
The PD mode demonstrated a bias of 13.0% for pediatric microsamples in the PLT-F channel. The automated method exhibited significantly lower imprecision and reduced turnaround times compared to manual methods.

Conclusions:

The automated microsample method provides a low-volume, efficient alternative for thrombocyte measurement.
This method demonstrates utility and potential for application in pediatric sample analysis.