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Updated: Jun 17, 2025
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
Published on: November 21, 2018
Chromatin Immunoprecipitation in Adipose Tissue and Adipocytes: How to Proceed and Optimize the Protocol for
Céline Jouffe1,2, Kenneth A Dyar3,4, Nina Henriette Uhlenhaut5,6
1Institute for Diabetes and Cancer, Helmholtz Zentrum Muenchen, Neuherberg, Germany. celine.jouffe@helmholtz-munich.de.
Abstract:
Chromatin immunoprecipitation (ChIP) coupled to qPCR or sequencing is a crucial experiment to determine direct transcriptional regulation under the control of specific transcriptional factors or co-regulators at loci-specific or pan-genomic levels.Here we provide a reliable method for processing ChIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipitation, and DNA purification. We also discuss critical steps for optimizing the experiment to perform a successful ChIP in lipid-rich cells/tissues.
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