Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbiome of the Eye

Microbiome of the Eye

The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Objective evaluation of clinical outcomes of laparoscopy-assisted pylorus-preserving gastrectomy for middle-third early gastric cancer.

BMC cancer·2019

Same author

miR‑767‑5p inhibits glioma proliferation and metastasis by targeting SUZ12.

Oncology reports·2019

Same author

Predicting Essential Proteins by Integrating Network Topology, Subcellular Localization Information, Gene Expression Profile and GO Annotation Data.

IEEE/ACM transactions on computational biology and bioinformatics·2019

Same author

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of the Volatile Oil of Cichorium Glandulosum Boiss et Huet and its Effects on Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Medical science monitor : international medical journal of experimental and clinical research·2019

Same author

Experimental study on healthy volunteers based on magnetic induction brain edema monitoring system.

Technology and health care : official journal of the European Society for Engineering and Medicine·2019

Same author

Tumor Chemo-Radiotherapy with Rod-Shaped and Spherical Gold Nano Probes: Shape and Active Targeting Both Matter.

Theranostics·2019

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

Assembling Retinal Organoids with Microglia.

Jia Xu¹, Si-Jian Yu¹, Zi-Bing Jin²

¹Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University.

Journal of Visualized Experiments : Jove

|August 14, 2024

Summary

This summary is machine-generated.

Researchers developed a novel retinal organoid model incorporating human stem cell-derived microglia. This advancement enables better study of retinal diseases and drug screening by including essential immune cells missing in previous models.

More Related Videos

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling

Published on: July 26, 2024

Derivation of a Human Brain Organoid with Microglia Development

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

Related Experiment Videos

Last Updated: Jun 20, 2026

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling

Generation of Human Microglia to Combine Them with Retinal Organoids for Improved Disease Modeling

Published on: July 26, 2024

Derivation of a Human Brain Organoid with Microglia Development

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

Area of Science:

* Stem cell biology
* Neuroscience
* Immunology

Background:

* Human retinal organoids (ROs) are crucial for studying retinal diseases but lack microglia, essential immune cells.
* Microglia (MG) are unique macrophages in the retina and CNS, vital for immunity.
* The absence of microglia in ROs limits understanding of their role in retinal disease pathogenesis.

Purpose of the Study:

* To establish a microglia-incorporated retinal organoid model.
* To create a more accurate platform for studying retinal diseases.
* To facilitate drug screening for retinal and CNS disorders.

Main Methods:

* Differentiation of microglia from human stem cells.
* Co-culture of differentiated microglia with retinal organoids in early stages.
* Development of a microglia-integrated retinal organoid system.

Main Results:

* Successful construction of a co-cultured retinal organoid model with human stem cell-derived microglia.
* Integration of microglia into the retinal organoid structure.
* Creation of an optimized platform for disease modeling and drug screening.

Conclusions:

* The developed model overcomes the limitation of lacking microglia in traditional retinal organoids.
* This microglia-incorporated model offers a powerful tool for investigating retinal disease mechanisms.
* It provides an enhanced platform for therapeutic development and drug discovery in ophthalmology and neurology.