Simultaneously performing Taylor dispersion analysis with fluorimetry, photometry, and contactless conductometry at...

Area of Science:

• Analytical Chemistry
• Physical Chemistry
• Biophysical Chemistry

Background:

• Taylor dispersion analysis (TDA) is a key technique for determining hydrodynamic radius (R_H).
• Existing TDA systems can be limited in detection capabilities and versatility.
• Simultaneous multi-detection enhances analytical power for complex samples.

Purpose of the Study:

• To develop and validate a versatile TDA system with integrated 3-in-1 detectors.
• To compare the performance of different detection methods (fluorimetry, photometry, conductometry) within the TDA framework.
• To evaluate the utility of capillary electrophoresis TDA (CE-TDA) for improved accuracy and mixture analysis.

Main Methods:

• Construction of a novel TDA system featuring a flow-through injection device and 3D-printed 3-in-1 detectors (fluorimetry, photometry, contactless conductometry).
• Analysis of bovine serum albumin (BSA) using pulse mode TDA and CE-TDA with varying buffer conditions.
• Application of the system to CE-TDA of charged saccharides and resolution of mixed signals.

Main Results:

• Fluorimetry (FD) and photometry (AD) provided consistent R_H values, while contactless conductometry (C⁴D) showed deviations, particularly in phosphate buffers due to BSA self-association.
• CE-TDA significantly improved the accuracy of C⁴D measurements compared to pulse mode TDA.
• The system successfully resolved complex mixtures, demonstrating the ability to separate overlapping signals using combined detection methods.

Conclusions:

• The developed versatile TDA system with 3-in-1 detection is a powerful tool for accurate R_H determination.
• CE-TDA enhances the reliability of TDA, especially when using conductometric detection.
• The integrated detection approach facilitates the analysis of complex samples and mixtures, increasing sample throughput.