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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Highly enhanced Hg2+ detection using optimized DNA and a double coffee ring effect-based SERS map.

Joohyung Park1, Kyunghwan Chai1, Woong Kim2

  • 1Department of Biomechatronics Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

Biosensors & Bioelectronics
|August 14, 2024
PubMed
Summary
This summary is machine-generated.

A new surface-enhanced Raman scattering (SERS) sensor effectively detects mercury ions (Hg2+) using optimized DNA probes and the coffee ring effect. This highly sensitive method offers reliable environmental and food safety monitoring.

Keywords:
Coffee ring effectDNA optimizationHg ionReal sampleSERS map

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Mercury ions (Hg2+) are highly toxic heavy metals posing significant risks to human health and ecosystems.
  • Bioaccumulation of mercury through the food chain necessitates sensitive detection methods under realistic conditions.

Purpose of the Study:

  • To develop a highly sensitive and selective surface-enhanced Raman scattering (SERS) sensor for mercury ion (Hg2+) detection.
  • To create a user-friendly method for assessing mercury contamination in environmental and food samples.

Main Methods:

  • Designed a selective DNA probe targeting Hg2+ via a thymine-thymine mismatch.
  • Utilized the double coffee ring effect to concentrate the DNA probe, enhancing SERS signals.
  • Employed SERS spectroscopy for quantitative analysis and created pizza-shaped SERS mapping images.

Main Results:

  • Achieved exceptional sensitivity with a low detection limit of 208.71 fM for Hg2+.
  • Demonstrated superior selectivity for Hg2+ over other metal ions.
  • Successfully detected Hg2+ in real-world samples including drinking water, tap water, canned tuna, and tuna sashimi.

Conclusions:

  • The developed SERS sensor provides a promising, reliable, and user-friendly approach for sensitive Hg2+ detection.
  • This method has significant potential for environmental monitoring and ensuring food safety by detecting mercury contamination.