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Chlormethine in small doses as immunostimulator--LPS synergism
Archivum Immunologiae Et Therapiae Experimentalis
|January 1, 1985
Summary
Chlormethine (Nitrogranulogen, Ntg) enhances immune responses in rabbits, boosting T and B cell counts and improving neutrophil activity. It also reduced fever duration when combined with LPS.
Area of Science:
- Immunology
- Pharmacology
Background:
- Chlormethine (Nitrogranulogen, Ntg) is a chemotherapeutic agent.
- Lipopolysaccharide (LPS) can induce fever in rabbits, mimicking inflammatory conditions.
Purpose of the Study:
- To investigate the immunomodulatory effects of chlormethine (Ntg) in normothermic and feverish rabbits.
- To assess the impact of Ntg on various immune parameters, including lymphocyte counts, antibody production, and neutrophil function.
Main Methods:
- Rabbits were treated with Ntg (1 µg/kg and 10 µg/kg) intravenously.
- Immune indices such as T and B cell numbers, IgM-producing cells, serum IgG, neutrophil killing activity, and phagocytosis were measured at various time points.
- Rabbits were also treated with Ntg in combination with LPS to study its effect on fever and immune response.
Main Results:
- Ntg significantly increased T and B lymphocytes, IgM-producing cells, serum IgG, neutrophil killing activity, and phagocytosis in normothermic rabbits.
- In LPS-induced fever models, Ntg shortened fever duration and synergistically enhanced T lymphocytes, IgG levels, neutrophil activity, and bacterial phagocytosis.
Conclusions:
- Chlormethine (Ntg) exhibits significant immunostimulatory properties in rabbits.
- Ntg demonstrates potential as an immunomodulator, particularly in enhancing cellular and humoral immunity and antimicrobial activity.