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Updated: Jun 17, 2025
MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
Exploration of microRNAs as transcriptional regulator in mumps virus infection through computational studies
Mubashir Hassan1, Saba Shahzadi1, Muhammad Shahzad Iqbal2
1The Steve and Cindy Rasmussen Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA.
Abstract:
Mumps is a common childhood infection caused by the mumps virus (MuV). Aseptic meningitis and encephalitis are usual symptoms of mumps together with orchitis and oophoritis that can arise in males and females, respectively. We have used computational tools: RNA22, miRanda and psRNATarget to predict the microRNA-mRNA binding sites to find the putative microRNAs playing role in the host response to mumps virus infection. Our computational studies indicate that hsa-mir-3155a is most likely involved in mumps infection. This was further investigated by the prediction of binding sites of hsa-mir-3155a to the MuV genome. Additionally, structure prediction using MC-Fold and MC-Sym, respectively has been applied to predict the 3D structures of miRNA and mRNA. The miRNA-mRNA interaction profile between has been confirmed through molecular docking simulation studies. Taken together, the putative miRNA (hsa_miR_6794_5p) has been found to be most likely involved in the regulation of transcriptional activity in the MuV infection.
Insights
Computational analysis suggests hsa-mir-3155a plays a role in mumps virus (MuV) infection. This microRNA may regulate transcriptional activity during MuV infection, offering insights into host responses.
Area of Science:
- Virology
- Molecular Biology
- Bioinformatics
Background:
- Mumps, caused by the mumps virus (MuV), is a common childhood infection.
- Mumps can lead to complications such as aseptic meningitis, encephalitis, orchitis, and oophoritis.
Purpose of the Study:
- To identify microRNAs involved in the host response to mumps virus infection using computational tools.
- To investigate the potential role of specific microRNAs in regulating MuV infection.
Main Methods:
- Utilized computational tools (RNA22, miRanda, psRNATarget) to predict microRNA-mRNA binding sites.
- Predicted 3D structures of microRNA and mRNA using MC-Fold and MC-Sym.
- Confirmed miRNA-mRNA interactions through molecular docking simulations.
Main Results:
- Identified hsa-mir-3155a as a putative microRNA involved in mumps infection.
- Predicted binding sites of hsa-mir-3155a within the MuV genome.
- Confirmed miRNA-mRNA interactions and suggested hsa_miR_6794_5p involvement in transcriptional regulation during MuV infection.
Conclusions:
- hsa-mir-3155a is likely involved in the host response to mumps virus infection.
- The microRNA hsa_miR_6794_5p may play a regulatory role in transcriptional activity during MuV infection.
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