Transcriptomic analysis for the gamma-ray-induced sweetpotato mutants with altered stem growth pattern.
Hyeong-Un Lee1,2, Sangrea Shim3, Mi Nam Chung2
1Department of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Frontiers in Genetics
|August 15, 2024
Summary
Gamma radiation induces genetic variation in sweetpotato, altering stem development. Key genes like PIF4 and SAUR were upregulated, influencing auxin signaling and stem growth in mutants.
Area of Science:
- Plant genomics
- Crop mutation breeding
Background:
- Sweetpotato breeding faces physiological and genomic challenges.
- Gamma radiation is a novel tool for inducing genetic variation in crops.
Purpose of the Study:
- To analyze transcriptomic changes in gamma ray-induced sweetpotato mutants with altered stem development.
- To understand the molecular mechanisms underlying gamma radiation's effect on sweetpotato stem growth.
Main Methods:
- RNA sequencing was employed to identify gene expression changes.
- Comparative transcriptomic analysis between mutant and wild-type sweetpotato ('Tongchaeru').
Main Results:
- Identified 8,931 upregulated and 6,901 downregulated genes in mutants.
- Observed upregulation of auxin-responsive SMALL AUXIN UP RNA (SAUR) and PHYTOCHROME INTERACTING FACTOR 4 (PIF4) genes.
- Found upregulation of stem elongation genes and those in auxin and gibberellin signaling pathways.
Conclusions:
- Gamma ray-induced mutations modulate auxin-dependent stem development via PIF4, SAUR, gibberellin, and ethylene signaling.
- The study provides insights into sweetpotato stem growth regulation for genomics-assisted breeding.
Related Concept Videos
02:13Overview of Transposition and Recombination
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
02:11Reporter Genes
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Commonly used reporter...
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...