Facile synthesis of copper carbonate analog with peroxidase-like activity for colorimetric detection of isoniazid

In this article, copper carbonate analog with good peroxidase-like activity was successfully synthesized for the first time via a simple co-precipitation of CuSO₄▪5H₂O and Na₂CO₃. The obtained copper carbonate analog exhibited excellent intrinsic peroxidase-like activity towards a classical peroxidase substrate of 3, 3', 5, 5' -tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H₂O₂) under an acidic environment. The study of the catalytic mechanism confirmed that the hydroxyl radical produced from the decomposition of H₂O₂ is the main reactive oxygen species responsible for the catalytic oxidation of TMB to oxTMB. Moreover, results from kinetic parameter analysis indicated that H₂O₂ was more easily and/or likely to attach to the copper carbonate analog than TMB. Subsequently, the effects of experimental conditions (buffer pH, temperature, and incubation time) on the catalytic activity of the copper carbonate analog were also optimized. Finally, a copper carbonate analog-based colorimetric sensor was developed to determine isoniazid. Under the optimal conditions, the linear range for isoniazid was as broad as 0-178.6 μM, and the detection limit was as low as 8.47 μM. The spiked recoveries of isoniazid in normal human serum has been observed in the range of 94.8%-105.5 %. This strategy focuses on the development of a green, cost-efficient peroxidase mimic with high activity, good biocompatibility, and a simple synthesis process.